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Solid phase synthesis and biological activity of rat relaxin

Wade, J.D., Lin, F., Talbo, G., Otvos Jr., L., Tan, Y.Y. and Tregear, G.W. (1996). Solid phase synthesis and biological activity of rat relaxin. Biomedical Peptides, Proteins & Nucleic Acids,2(3):89-92.

Document type: Journal Article
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Title Solid phase synthesis and biological activity of rat relaxin
Author Wade, J.D.
Lin, F.
Talbo, G.
Otvos Jr., L.
Tan, Y.Y.
Tregear, G.W.
Journal Name Biomedical Peptides, Proteins & Nucleic Acids
Publication Date 1996
Volume Number 2
Issue Number 3
ISSN 1353-8616   (check CDU catalogue open catalogue search in new window)
Start Page 89
End Page 92
Total Pages 4
Place of Publication United Kingdom
Publisher Mayflower Worldwide Ltd.
Language English
Field of Research 1115 - Pharmacology and Pharmaceutical Sciences
Abstract The peptide hormone relaxin was isolated in good yield from the ovaries of the pregnant rodent Rattus rattus using a simplified purification schedule. It was subjected to comprehensive chemical characterization to confirm both its purity and predicted composition. The peptide was also chemically synthesized by the solid phase procedure. The two chains comprising the hormone were each assembled by the Boc-polystyrene method and, following conventional purification, combined in solution to form the single intramolecular and two intermolecular disulfide bonds. Following purification, the synthetic rat relaxin was fully chemically characterized and shown to be indistinguishable from the native peptide including by secondary structure analysis using circular dichroism spectroscopy. The native and synthetic rat relaxins were shown to be equally biologically active in the in vitro rat uterine relaxation assay and had pEC50 values that were comparable to synthetic human H2 relaxin.
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Created: Mon, 08 Dec 2014, 15:04:32 CST by Yean Tan