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Immunological response mounted by Aboriginal Australians living in the Northern Territory of Australia against Streptococcus pyogenes serum opacity factor

Gillen, C. M., Towers, Rebecca J., McMillan, D. J., Delvecchio, A., Sriprakash, Kadaba S., Currie, Bart J., Kreikemeyer, B., Chhatwal, G. S. and Walker, M. J. (2002). Immunological response mounted by Aboriginal Australians living in the Northern Territory of Australia against Streptococcus pyogenes serum opacity factor. Microbiology,148:169-178.

Document type: Journal Article
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Title Immunological response mounted by Aboriginal Australians living in the Northern Territory of Australia against Streptococcus pyogenes serum opacity factor
Author Gillen, C. M.
Towers, Rebecca J.
McMillan, D. J.
Delvecchio, A.
Sriprakash, Kadaba S.
Currie, Bart J.
Kreikemeyer, B.
Chhatwal, G. S.
Walker, M. J.
Journal Name Microbiology
Publication Date 2002
Volume Number 148
ISSN 1350-0872   (check CDU catalogue open catalogue search in new window)
Start Page 169
End Page 178
Total Pages 10
Place of Publication UK
Publisher Society for General Microbiology
Language English
Field of Research 320000 Medical and Health Sciences
Abstract Streptococcus pyogenes (Group A streptococcus) interacts with host fibronectin via a number of distinct surface components. The streptococcal serum opacity factor (SOF) is a cell-surface protein of S. pyogenes which causes opalescence of human serum and mediates bacterial binding to fibronectin. In this study, hexahistidyl-tagged fusion proteins encompassing full-length SOF, and domains of SOF encompassing opacity factor activity and fibronectin-binding regions, were used in the characterization of the Aboriginal immune response to SOF. Anti-SOF serum IgG responses were found to be significantly higher (P>0.0001) in Aboriginal adults and children when compared to a non-Aboriginal adult group. The Aboriginal immune response against the fibronectin-binding region of SOF was significantly reduced when compared to the response against the whole SOF protein and N-terminal domains examined in this study (P>0.001). This pattern of immune response was also observed in rabbits immunized with recombinant SOF. Comparison of the deduced amino acid sequence of SOF from a number of common Australian isolates with other SOF sequences revealed that the N-terminus of SOF exhibits sequence similarity values ranging from 42.9% to 96.5%. The C-terminus containing the fibronectin-binding domain and membrane-spanning regions was more highly conserved, exhibiting sequence similarity values ranging from 84.6% to 100% within the fibronectin-binding repeats. These data suggest that the immune response against SOF is directed toward the variable N-terminus of the SOF protein. Phylogenetic analysis indicated that the sof genes of S. pyogenes do not exhibit geographical variation.
Keywords Aboriginal
Northern Territory
pyogenes
Streptococcus
Additional Notes 2130 (Journal) DA - 20020109IS - 1350-0872 (Print)LA - engPT - Journal ArticlePT - Research Support, Non-U.S. Gov'tRN - 0 (Adhesins, Bacterial)RN - 0 (Antibodies, Bacterial)RN - 0 (Bacterial Proteins)RN - 0 (Carrier Proteins)RN - 0 (Recombinant Proteins)RN - 0 (fibronectin-binding protein, Bacteria)RN - 0 (opacity factor)RN - EC 3.4.- (Peptide Hydrolases)SB - IM
 
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Created: Mon, 17 Dec 2007, 09:02:11 CST