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Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea

Baker, Anthony Baker, Pearson, Talima, Price, Erin P., Dale, Julia, Keim, Paul, Hornstra, Heidie, Greenhill, Andrew, Padilla, Gabriel and Warner, Jeffrey (2011). Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea. PLoS One,6(3):e18343-1-e18343-6.

Document type: Journal Article
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IRMA ID 75039815xPUB519
Title Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea
Author Baker, Anthony Baker
Pearson, Talima
Price, Erin P.
Dale, Julia
Keim, Paul
Hornstra, Heidie
Greenhill, Andrew
Padilla, Gabriel
Warner, Jeffrey
Journal Name PLoS One
Publication Date 2011
Volume Number 6
Issue Number 3
ISSN 1932-6203   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-79953672651
Start Page e18343-1
End Page e18343-6
Total Pages 6
Place of Publication United States
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract Background:
The island of New Guinea is located midway between the world's two major melioidosis endemic regions of Australia and Southeast Asia. Previous studies in Papua New Guinea have demonstrated autochthonous melioidosis in Balimo, Western province. In contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales.

Methodology/Principal Findings:
We employed molecular typing techniques to determine the phylogenetic relationships of these isolates to each other and to others worldwide to aid in understanding the origins of the Papua New Guinean isolates. Multi-locus sequence typing of the 39 isolates resolved three unique sequence types. Phylogenetic reconstruction and Structure analysis determined that all isolates were genetically closer to those from Australia than those from Southeast Asia. Gene cluster analysis however, identified a Yersinia-like fimbrial gene cluster predominantly found among Burkholderia pseudomallei derived from Southeast Asia. Higher resolution VNTR typing and phylogenetic reconstruction of the Balimo isolates resolved 24 genotypes with long branch lengths. These findings are congruent with long term persistence in the region and a high level of environmental stability.

Conclusions/Significance:
Given that anthropogenic influence has been hypothesized as a mechanism for the dispersal of B. pseudomallei, these findings correlate with limited movement of the indigenous people in the region. The palaeogeographical and anthropogenic history of Australasia and the results from this study indicate that New Guinea is an important region for the further study of B. pseudomallei origins and dissemination.
DOI http://dx.doi.org/10.1371/journal.pone.0018343   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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