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Identification of a homologue of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var. hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model

Harumal, Pearly, Morgan, M., Walton, Shelley F., Holt, Deborah C., Rode, J., Arlian, L. G., Currie, Bart J. and Kemp, David J. (2003). Identification of a homologue of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var. hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model. American Journal of Tropical Medicine and Hygiene,68(1):54-60.

Document type: Journal Article
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Title Identification of a homologue of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var. hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model
Author Harumal, Pearly
Morgan, M.
Walton, Shelley F.
Holt, Deborah C.
Rode, J.
Arlian, L. G.
Currie, Bart J.
Kemp, David J.
Journal Name American Journal of Tropical Medicine and Hygiene
Publication Date 2003
Volume Number 68
Issue Number 1
ISSN 1476-1645   (check CDU catalogue open catalogue search in new window)
Start Page 54
End Page 60
Total Pages 7
Place of Publication USA
Publisher The American Society of Tropical Medicine and Hygiene
Language English
Field of Research 1117 - Public Health and Health Services
HERDC Category C1 - Journal Article (DEST)
Abstract Sarcoptes scabiei (“itchmite”) causes scabies, a disease of considerable human and veterinary significance. Little work has been done at the molecular level because of the difficulty of obtaining mites. We have used mites in skin from the bedding of crusted scabies patients for the construction of a library of 105 cDNAs from S. scabiei var. hominis cloned in the vector pGEX4T-2. We describe the isolation by immunoscreening of 2 clones, one of which (Ssag1) is homologous to and cross-reactive with the house dust mite Euroglyphus maynei allergen M-177, an apolipoprotein from hemolymph. Immunohistochemistry revealed that it is located around the internal organs and cuticle of the mite and in eggs. Although it was not found to be protective in a challenge trial, the rabbits did not exhibit typical crust characteristics. This work shows that it is now possible to conduct such challenge trials withcloned scabies antigens.
Keywords parasitology
Rabbits
Sarcoptes scabiei
Additional Notes 3576 (Journal)
Description for Link Link to abstract
URL http://www.ncbi.nlm.nih.gov/pubmed/12556149
 
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Created: Mon, 17 Dec 2007, 09:02:11 CST