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Evolution of sfbI encoding Streptococcal fibronectin-binding protein I: horizontal genetic transfer and gene mosaic structure

Towers, Rebecca J., Fagan, Peter K., Talay, Susanne R., Currie, Bart J., Sriprakash, Kadaba S., Walker, Mark J. and Chhatwal, Gursharan S. (2003). Evolution of sfbI encoding Streptococcal fibronectin-binding protein I: horizontal genetic transfer and gene mosaic structure. Journal of Clinical Microbiology,41(12):5398-5406.

Document type: Journal Article
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Title Evolution of sfbI encoding Streptococcal fibronectin-binding protein I: horizontal genetic transfer and gene mosaic structure
Author Towers, Rebecca J.
Fagan, Peter K.
Talay, Susanne R.
Currie, Bart J.
Sriprakash, Kadaba S.
Walker, Mark J.
Chhatwal, Gursharan S.
Journal Name Journal of Clinical Microbiology
Publication Date 2003
Volume Number 41
Issue Number 12
ISSN 1098-660X   (check CDU catalogue  open catalogue search in new window)
Start Page 5398
End Page 5406
Total Pages 9
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 0605 - Microbiology
0707 - Veterinary Sciences
1108 - Medical Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Streptococcal fibronectin-binding protein is an important virulence factor involved in colonization and invasion of epithelial cells and tissues by Streptococcus pyogenes. In order to investigate the mechanisms involved in the evolution of sfbI, the sfbI genes from 54 strains were sequenced. Thirty-four distinct alleles were identified. Three principal mechanisms appear to have been involved in the evolution of sfbI. The amino-terminal aromatic amino acid-rich domain is the most variable region and is apparently generated by intergenic recombination of horizontally acquired DNA cassettes, resulting in a genetic mosaic in this region. Two distinct and divergent sequence types that shared only 61 to 70% identity were identified in the central proline-rich region, while variation at the 3' end of the gene is due to deletion or duplication of defined repeat units. Potential antigenic and functional variabilities in SfbI imply significant selective pressure in vivo with direct implications for the microbial pathogenesis of S. pyogenes.
Keywords Fibronectin
Gene Mosaic
Protein
Streptococcal
DOI http://dx.doi.org/10.1128/JCM.41.12.5398-5406.2003   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright by the American Society for Microbiology


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