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Increased plasma arginase activity in human sepsis: association with increased circulating neutrophils

Darcy, Christabelle J., Woodberry, Tonia, Davis, Joshua S., Piera, Kim A., McNeil, Yvette R., Chen, Youwei, Yeo, Tsin W., Weinberg, J. B. and Anstey, Nicholas M. (2014). Increased plasma arginase activity in human sepsis: association with increased circulating neutrophils. Clinical Chemistry and Laboratory Medicine,52(4):573-581.

Document type: Journal Article
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IRMA ID cmartelxPUB93
Title Increased plasma arginase activity in human sepsis: association with increased circulating neutrophils
Author Darcy, Christabelle J.
Woodberry, Tonia
Davis, Joshua S.
Piera, Kim A.
McNeil, Yvette R.
Chen, Youwei
Yeo, Tsin W.
Weinberg, J. B.
Anstey, Nicholas M.
Journal Name Clinical Chemistry and Laboratory Medicine
Publication Date 2014
Volume Number 52
Issue Number 4
ISSN 1437-4331   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84896791065
Start Page 573
End Page 581
Total Pages 10
Place of Publication Germany
Publisher Walter de Gruyter GmbH & Co.
HERDC Category C1 - Journal Article (DIISR)
Abstract BACKGROUND:
The pathophysiology of sepsis is incompletely understood. Impaired bioavailability of L-arginine, the substrate for NO synthesis, is linked to sepsis severity, and plasma arginase has been linked to hypoargininemia in other disease states. Circulating neutrophils are increased in sepsis and constitutively express arginase. We investigated whether plasma arginase activity is increased in human sepsis and whether this is associated with neutrophil numbers and activation.
METHODS:
We used HPLC and a radiometric assay to evaluate plasma amino acid concentrations and plasma arginase activity. The relationships between plasma arginase activity, neutrophil count, neutrophil activity and plasma L-arginine and arginine metabolites were evaluated in 44 sepsis patients and 25 controls.
RESULTS:
Plasma arginase activity was increased in sepsis patients, correlated with neutrophil count (r=0.44; p=0.003), but was independent of sepsis severity (SOFA or APACHE II score). Plasma HNP1-3 correlated with neutrophil count (r=0.31; p=0.04), was elevated in shock (median 180 ng/mL vs. 83 ng/mL sepsis without shock, p=0.0006) and correlated with SOFA score. Sepsis patients with high neutrophil counts had significantly higher plasma HNP1-3 and arginase activity and lower plasma L-arginine concentrations than those with lower neutrophil counts and controls.
CONCLUSIONS:
Plasma arginase activity, potentially derived in part from neutrophil activation, is elevated in sepsis, and may contribute to impaired bioavailability of L-arginine in sepsis.
Keywords Hypoargininema
L-arginine
Plasma arginase activity
Sepsis
DOI http://dx.doi.org/10.1515/cclm-2013-0698   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Description for Link Link to publisher's version
URL http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431981/
 
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