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Isolation and characterisation of 16 microsatellite markers for the endangered Gould's long-eared bat (Nyctophilus gouldi) and cross-amplification in the lesser long-eared bat (N. geoffroyi)

Fuller, Nicholas C., Carthew, Susan M. and Cooper, Steven J. B. (2014). Isolation and characterisation of 16 microsatellite markers for the endangered Gould's long-eared bat (Nyctophilus gouldi) and cross-amplification in the lesser long-eared bat (N. geoffroyi). Conservation Genetics Resources,6(3):519-522.

Document type: Journal Article
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IRMA ID 75039815xPUB302
Title Isolation and characterisation of 16 microsatellite markers for the endangered Gould's long-eared bat (Nyctophilus gouldi) and cross-amplification in the lesser long-eared bat (N. geoffroyi)
Author Fuller, Nicholas C.
Carthew, Susan M.
Cooper, Steven J. B.
Journal Name Conservation Genetics Resources
Publication Date 2014
Volume Number 6
Issue Number 3
ISSN 1877-7252   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84905657691
Start Page 519
End Page 522
Total Pages 4
Place of Publication Netherlands
Publisher Springer Netherlands
HERDC Category C1 - Journal Article (DIISR)
Abstract Sixteen microsatellite markers were developed for use on two species of long-eared bats (Nyctophilus). 454 pyrosequencing of genomic DNA was conducted on N. gouldi which is listed as endangered in South Australia. Fifteen loci successfully amplified on N. gouldi while nine cross-amplified for use on N. geoffroyi. Two populations from south-eastern Australia were genotyped for each species, comprising 91 individuals for N. gouldi and 70 individuals for N. geoffroyi. There was no evidence of linkage disequilibrium and all loci displayed Hardy–Weinberg equilibrium except Nyg19 and Nyg39 which displayed evidence of null alleles in both N. geoffroyi populations. These markers will prove valuable in assessing connectivity between endangered populations of N. gouldi, and facilitate a comparative investigation into the impacts of habitat fragmentation on two vespertilionids.
Keywords Nyctophilus
Chiroptera
Microsatellites
454 Pyrosequencing
DOI http://dx.doi.org/10.1007/s12686-014-0165-0   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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