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Burkholderia pseudomallei Isolates from Sarawak, Malaysian Borneo, Are Predominantly Susceptible to Aminoglycosides and Macrolides

Podin, Yuwana, Sarovich, Derek S., Price, Erin P., Kaestli, Mirjam, Mayo, Mark, Hii, KingChing, Ngian, HieUng, Wong, See-Chang, Wong, IngTien, Wong, JinShyan, Mohan, Anand, Ooi, MongHow, Fam, TemLom, Wong, Jack, Tuanyok, Apichai, Keim, Paul, Giffard, Philip M. and Currie, Bart J. (2014). Burkholderia pseudomallei Isolates from Sarawak, Malaysian Borneo, Are Predominantly Susceptible to Aminoglycosides and Macrolides. Antimicrobial Agents and Chemotherapy,58(1):162-166.

Document type: Journal Article
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IRMA ID 11035xPUB75
Title Burkholderia pseudomallei Isolates from Sarawak, Malaysian Borneo, Are Predominantly Susceptible to Aminoglycosides and Macrolides
Author Podin, Yuwana
Sarovich, Derek S.
Price, Erin P.
Kaestli, Mirjam
Mayo, Mark
Hii, KingChing
Ngian, HieUng
Wong, See-Chang
Wong, IngTien
Wong, JinShyan
Mohan, Anand
Ooi, MongHow
Fam, TemLom
Wong, Jack
Tuanyok, Apichai
Keim, Paul
Giffard, Philip M.
Currie, Bart J.
Journal Name Antimicrobial Agents and Chemotherapy
Publication Date 2014
Volume Number 58
Issue Number 1
ISSN 0066-4804   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84891516945
Start Page 162
End Page 166
Total Pages 5
Place of Publication Washington, United States of America
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DIISR)
Abstract Melioidosis is a potentially fatal disease caused by the saprophytic bacterium Burkholderia pseudomallei. Resistance to gentamicin is generally a hallmark of B. pseudomallei, and gentamicin is a selective agent in media used for diagnosis of melioidosis. In this study, we determined the prevalence and mechanism of gentamicin susceptibility found in B. pseudomallei isolates from Sarawak, Malaysian Borneo. We performed multilocus sequence typing and antibiotic susceptibility testing on 44 B. pseudomallei clinical isolates from melioidosis patients in Sarawak district hospitals. Whole-genome sequencing was used to identify the mechanism of gentamicin susceptibility. A novel allelic-specific PCR was designed to differentiate gentamicin-sensitive isolates from wild-type B. pseudomallei. A reversion assay was performed to confirm the involvement of this mechanism in gentamicin susceptibility. A substantial proportion (86%) of B. pseudomallei clinical isolates in Sarawak, Malaysian Borneo, were found to be susceptible to the aminoglycoside gentamicin, a rare occurrence in other regions where B. pseudomallei is endemic. Gentamicin sensitivity was restricted to genetically related strains belonging to sequence type 881 or its single-locus variant, sequence type 997. Whole-genome sequencing identified a novel nonsynonymous mutation within amrB, encoding an essential component of the AmrAB-OprA multidrug efflux pump. We confirmed the role of this mutation in conferring aminoglycoside and macrolide sensitivity by reversion of this mutation to the wild-type sequence. Our study demonstrates that alternative B. pseudomallei selective media without gentamicin are needed for accurate melioidosis laboratory diagnosis in Sarawak. This finding may also have implications for environmental sampling of other locations to test for B. pseudomallei endemicity.
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