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A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Pickering, Janessa, Binks, Michael J., Beissbarth, Jemima, Hare, Kim M., Kirkham, Lea-Ann S. and Smith-Vaughan, Heidi C. (2014). A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus. Journal of Clinical Microbiology,52(2):663-667.

Document type: Journal Article
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IRMA ID cmartelxPUB137
Title A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
Author Pickering, Janessa
Binks, Michael J.
Beissbarth, Jemima
Hare, Kim M.
Kirkham, Lea-Ann S.
Smith-Vaughan, Heidi C.
Journal Name Journal of Clinical Microbiology
Publication Date 2014
Volume Number 52
Issue Number 2
ISSN 0095-1137   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84893087607
Start Page 663
End Page 667
Total Pages 5
Place of Publication United States of America
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DIISR)
Abstract We have developed a PCR–high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.
DOI http://dx.doi.org/10.1128/JCM.02191-13   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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