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Quantification of Plasmodium falciparum Histidine-Rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients

Mikita, Kei, Thakur, Kiran, Anstey, Nicholas M., Piera, Kim A, Carlos, Pardo A., Weinberg, J. Brice, Mukemba, Jackson, Florence, Salvatore, Mwaikambo, Esther D., Granger, Donald L. and Sullivan, David J. (2014). Quantification of Plasmodium falciparum Histidine-Rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients. American Journal of Tropical Medicine and Hygiene,91(3):486-492.

Document type: Journal Article
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IRMA ID cmartelxPUB181
Title Quantification of Plasmodium falciparum Histidine-Rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients
Author Mikita, Kei
Thakur, Kiran
Anstey, Nicholas M.
Piera, Kim A
Carlos, Pardo A.
Weinberg, J. Brice
Mukemba, Jackson
Florence, Salvatore
Mwaikambo, Esther D.
Granger, Donald L.
Sullivan, David J.
Journal Name American Journal of Tropical Medicine and Hygiene
Publication Date 2014
Volume Number 91
Issue Number 3
ISSN 0002-9637   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84907016672
Start Page 486
End Page 492
Total Pages 7
Place of Publication United States of America
Publisher American Society of Tropical Medicine and Hygiene
HERDC Category C1 - Journal Article (DIISR)
Abstract A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker.
DOI http://dx.doi.org/10.4269/ajtmh.14-0210   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Description for Link Link to publisher's version
URL http://www.ajtmh.org/content/91/3/486
 
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