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Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma In vitro in response to non-typeable haemophilus influenzae

Pizzutto, Susan J., Yerkovich, Stephanie T., Upham, John, Hales, Belinda, Thomas, Wayne and Chang, Anne B. (2014). Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma In vitro in response to non-typeable haemophilus influenzae. PLoS One,9(8 - Article No. e104236).

Document type: Journal Article
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IRMA ID cspargoxPUB26
NHMRC Grant No. 1038415
Title Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma In vitro in response to non-typeable haemophilus influenzae
Author Pizzutto, Susan J.
Yerkovich, Stephanie T.
Upham, John
Hales, Belinda
Thomas, Wayne
Chang, Anne B.
Journal Name PLoS One
Publication Date 2014
Volume Number 9
Issue Number 8 - Article No. e104236
ISSN 1932-6203   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84905833828
Total Pages 10
Place of Publication United States of America
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract Chronic suppurative lung disease (CSLD) is characterized by the presence of a chronic wet or productive cough and recurrent lower respiratory infections. The aim of this study was to identify features of innate, cell-mediated and humoral immunity that may increase susceptibility to respiratory infections in children with CSLD. Because non-typeable Haemophilus influenzae (NTHi) is commonly isolated from the airways in CSLD, we examined immune responses to this organism in 80 age-stratified children with CSLD and compared their responses with 51 healthy control children. Cytokines involved in the generation and control of inflammation (IFN-γ, IL-13, IL-5, IL-10 at 72 hours and TNFα, IL-6, IL-10 at 24 hours) were measured in peripheral blood mononuclear cells challenged in vitro with live NTHi. We also measured circulating IgG subclass antibodies (IgG1 and IgG4) to two H. influenzae outer membrane proteins, P4 and P6. The most notable finding was that PBMC from children with CSLD produced significantly less IFN-γ in response to NTHi than healthy control children whereas mitogen-induced IFN-γ production was similar in both groups. Overall there were minor differences in innate and humoral immune responses between CSLD and control children. This study demonstrates that children with chronic suppurative lung disease have an altered systemic cell-mediated immune response to NTHi in vitro. This deficient IFN-γ response may contribute to increased susceptibility to NTHi infections and the pathogenesis of CSLD in children.
DOI http://dx.doi.org/10.1371/journal.pone.0104236   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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