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Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE

Mounsey, Kate E., Kearns, Therese M., Rampton, Melanie, Llewellyn, Stacey, King, Mallory, Holt, Deborah C., Currie, Bart J., Andrews, Ross M., Nutman, Thomas and McCarthy, James (2014). Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE. Acta Tropica,138:78-82.

Document type: Journal Article
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IRMA ID cspargoxPUB27
Title Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE
Author Mounsey, Kate E.
Kearns, Therese M.
Rampton, Melanie
Llewellyn, Stacey
King, Mallory
Holt, Deborah C.
Currie, Bart J.
Andrews, Ross M.
Nutman, Thomas
McCarthy, James
Journal Name Acta Tropica
Publication Date 2014
Volume Number 138
ISSN 0001-706X   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84904851871
Start Page 78
End Page 82
Total Pages 5
Place of Publication Netherlands
Publisher Elsevier BV
HERDC Category C1 - Journal Article (DIISR)
Abstract An approach to improve the diagnosis of Strongyloides stercoralis infection is the use of serologic assays utilising the NIE antigen from S. stercoralis, with good diagnostic sensitivity and excellent specificity reported. Detection of antibody eluted from dried blood spots (DBS) has shown utility in large-scale seroepidemiological studies for a range of conditions and is appealing for use with children where sample collection is difficult. We adapted an existing NIE-enzyme linked immunosorbent assay (ELISA) for the testing of strongyloides antibody response on DBS, and evaluated it in a population screening and mass drug administration programme (MDA) for strongyloidiasis conducted in an Australian indigenous community. Study participants were treated with 200 μg/kg ivermectin (>15 kg) or 3× 400 mg albendazole (<15 kg). The sensitivity of the NIE DBS-ELISA was determined by receiver operator characteristic (ROC) analysis to be 85.7%. A total of 214 DBS were collected from 184 participants across two screening and MDA encounters. A total of 27 of 164 participants (16.5%) tested positive for S. stercoralis NIE-DBS prior to MDA treatment, and 6 of 50 participants (12.0%) tested positive after treatment. These prevalence values are similar to those documented by standard serology in the same community. For 30 participants where a DBS was collected at both MDA 1 and 2, a significant decline in ELISA values was evident post treatment (0.12–0.02, p = 0.0012). These results are in agreement with previous studies documenting the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggest that collection of dried blood spots may be a useful approach for field diagnosis of S. stercoralis seroprevalence.
Keywords Strongyloides stercoralis
Serological diagnosis
Helminth infection
Indigenous health
DOI http://dx.doi.org/10.1016/j.actatropica.2014.07.007   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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