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A glutathione-dependent formaldehyde dehydrogenase gene of symbiotic cyanobacterium Anabaena azollae

Shaw, WH, Muirhead, A, Arioli, T and Plazinski, J (2001). A glutathione-dependent formaldehyde dehydrogenase gene of symbiotic cyanobacterium Anabaena azollae. Symbiosis,30(1):41-55.

Document type: Journal Article
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Title A glutathione-dependent formaldehyde dehydrogenase gene of symbiotic cyanobacterium Anabaena azollae
Author Shaw, WH
Muirhead, A
Arioli, T
Plazinski, J
Journal Name Symbiosis
Publication Date 2001
Volume Number 30
Issue Number 1
ISSN 0334-5114   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0035011118
Start Page 41
End Page 55
Total Pages 15
Place of Publication Philadelphia
Publisher Balaban
HERDC Category C1 - Journal Article (DEST)
Abstract A 3.2 kb fragment of an indigenous Anabaena azollae plasmid was isolated and fully sequenced. Sequence analysis identified an open reading frame of 1110-bp that showed high similarity (56-71%) with glutathione dependent formaldehyde dehydrogenase genes (gdfaldh) from various bacteria. The identity of the gene was confirmed by expressing the gene in Escherichia coli with a pET-32 (a) vector followed by enzyme assay. Adjacent to the gdfaldh a second gene was detected that showed high similarity (53%) with an S-formylglutathione hydrolase (fgh) gene from the methylotrophic bacterium Paracoccus denitrificans. The presence of gdfaldh and fgh-like genes adjacent to each other may suggest that the corresponding gene products interact in a common metabolic pathway involved in removing exogenous or endogenous formaldehyde.
Keywords anabaena azollae
azolla
symbiosis
plasmids
gdfaldh
alcohol-dehydrogenase
s-formylglutathione hydrolase
fatty-acid activation
esterase-d
paracoccus-denitrificans
escherichia-coli
plasmid
polymorphisms
recognition
mutagenesis
 
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Created: Fri, 12 Sep 2008, 08:35:25 CST by Administrator