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Susceptibility and intrinsic tolerance of Pseudomonas aeruginosa to selected plant volatile compounds

Cox, Sean D. and Markham, J. L. (2007). Susceptibility and intrinsic tolerance of Pseudomonas aeruginosa to selected plant volatile compounds. Journal of Applied Microbiology,103(4):930-936.

Document type: Journal Article
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Title Susceptibility and intrinsic tolerance of Pseudomonas aeruginosa to selected plant volatile compounds
Author Cox, Sean D.
Markham, J. L.
Journal Name Journal of Applied Microbiology
Publication Date 2007
Volume Number 103
Issue Number 4
ISSN 1364-5072   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-34848825600
Start Page 930
End Page 936
Total Pages 7
Publisher Blackwell Publishing
HERDC Category C1 - Journal Article (DEST)
Abstract Aims: To examine the causes for variations in sensitivity and intrinsic tolerance of Pseudomonas aeruginosa to plant volatile compounds. Methods and Results: Minimum inhibitory concentrations were determined for a selection of volatile phytochemicals against P. aeruginosa using a microdilution assay. Effects on growth were also assessed in 100-ml broth cultures. The two strains of P. aeruginosa included in the study exhibited intrinsic tolerance to all compounds, with the exception of carvacrol and trans-cinnamaldehyde. The protonophore carbonyl cyanide m-chlorophenylhydrazone increased P. aeruginosa sensitivity to all compounds except trans-cinnamaldehyde, implicating an ATP-dependent efflux mechanism in the observed tolerance. Outer membrane integrity following treatment with test compounds was assessed by measuring sensitization to detergents. Only carvacrol caused damage to the outer membrane of P. aeruginosa. Conclusions: The intrinsic tolerance of P. aeruginosa strains to plant volatile compounds is associated with an active efflux mechanism and the barrier function of the outer membrane. Significance and Impact of the Study: These findings offer an explanation for the intrinsic tolerance to plant volatile compounds exhibited by P. aeruginosa. The study also confirms that the outer membrane-permeabilizing action of carvacrol, previously reported for the gram-negative bacteria Escherichia coli and Salmonella, extends to monoterpene-tolerant strains of P. aeruginosa.
Keywords antimicrobial
essential oils
plant volatile compounds
Pseudomonas aeruginosa
tolerance
tea tree oil
pathogen bacillus-cereus
antimicrobial activity
outer-membrane
carvacrol
DOI http://dx.doi.org/10.1111/j.1365-2672.2007.03353.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Mon, 08 Sep 2008, 16:16:22 CST