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Stylosanthes is a host for several phytoplasmas, one of which shows unique 16S-23S intergenic spacer region heterogeneity

De La Rue, Stephanie J., Padovan, Anna C. and Gibb, Karen S. (2001). Stylosanthes is a host for several phytoplasmas, one of which shows unique 16S-23S intergenic spacer region heterogeneity. Journal of Phytopathology,149(10):613-619.

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Title Stylosanthes is a host for several phytoplasmas, one of which shows unique 16S-23S intergenic spacer region heterogeneity
Author De La Rue, Stephanie J.
Padovan, Anna C.
Gibb, Karen S.
Journal Name Journal of Phytopathology
Publication Date 2001
Volume Number 149
Issue Number 10
ISSN 0931-1785   (check CDU catalogue open catalogue search in new window)
Start Page 613
End Page 619
Total Pages 7
Place of Publication Berlin, Germany
Publisher Blackwell Publishingl
HERDC Category C1 - Journal Article (DEST)
Abstract Stylosanthes sp. exhibiting characteristic symptoms such as little leaf, witches' broom and floral abnormalities were collected from north Queensland and the Northern Territory, Australia. Previous studies have shown that sweet potato little leaf V4 (SPLL-V4), tomato big bud (TBB), stylosanthes little leaf (StLL) and pigeon pea little leaf (PLL) phytoplasmas are associated with this disease. The detection of an additional phytoplasma type, vigna little leaf (ViLL) is reported herein. The range and severity of symptoms expressed by affected plants is highly variable and is not associated with a particular phytoplasma type. Similarly, host plants infected with a complex of two phytoplasmas did riot have unique or more severe symptoms. Of the phytoplasmas associated with stylosanthes little leaf disease, StLL is unique because it lacks the tRNA(Ile) gene which is normally situated in the 16S-23S rRNA intergenic spacer region. This phytoplasma was shown to have a second operon containing the expected tRNA(Ile) gene in all StLL samples examined. Sequence analysis suggests that the two 16S rRNA genes amplified by polymerase chain reaction from StLL samples originate from the same phytoplasma. This the first report of a phytoplasma having ribosomal operons both with and without an intergenic tRNA(Ile) gene.
Keywords polymerase chain reaction
phytoplasma
stylosanthes
16s ribosomal-rna
pathogenic mycoplasmalike organisms
sequence heterogeneity
genes
differentiation
amplification
mollicutes
australia
symptoms
identity
DOI http://dx.doi.org/10.1111/j.1439-0434.2001.00683.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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