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Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus

Steinig, Eike J., Andersson, Patiyan, Harris, Simon R., Sarovich, Derek S., Manoharan, Anand, Coupland, Paul, Holden, Matthew T. G., Parkhill, Julian, Bentley, Stephen D., Robinson, D. Ashley and Tong, Steven Y. C. (2015). Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus. BMC Genomics,16(388).

Document type: Journal Article
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IRMA ID 11381xPUB70
NHMRC Grant No. 1065736
Title Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus
Author Steinig, Eike J.
Andersson, Patiyan
Harris, Simon R.
Sarovich, Derek S.
Manoharan, Anand
Coupland, Paul
Holden, Matthew T. G.
Parkhill, Julian
Bentley, Stephen D.
Robinson, D. Ashley
Tong, Steven Y. C.
Journal Name BMC Genomics
Publication Date 2015
Volume Number 16
Issue Number 388
ISSN 1471-2164   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84929322411
Total Pages 10
Place of Publication United Kingdom
Publisher BioMed Central Ltd.
HERDC Category C1 - Journal Article (DIISR)
Abstract Background
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-associated infection, but there is growing awareness of the emergence of multidrug-resistant lineages in community settings around the world. One such lineage is ST772-MRSA-V, which has disseminated globally and is increasingly prevalent in India. Here, we present the complete genome sequence of DAR4145, a strain of the ST772-MRSA-V lineage from India, and investigate its genomic characteristics in regards to antibiotic resistance and virulence factors.

Results

Sequencing using single-molecule real-time technology resulted in the assembly of a single continuous chromosomal sequence, which was error-corrected, annotated and compared to nine draft genome assemblies of ST772-MRSA-V from Australia, Malaysia and India. We discovered numerous and redundant resistance genes associated with mobile genetic elements (MGEs) and known core genome mutations that explain the highly antibiotic resistant phenotype of DAR4145. Staphylococcal toxins and superantigens, including the leukotoxin Panton-Valentinin Leukocidin, were predominantly associated with genomic islands and the phage φ-IND772PVL. Some of these mobile resistance and virulence factors were variably present in other strains of the ST772-MRSA-V lineage.

Conclusions

The genomic characteristics presented here emphasize the contribution of MGEs to the emergence of multidrug-resistant and highly virulent strains of community-associated MRSA. Antibiotic resistance was further augmented by chromosomal mutations and redundancy of resistance genes. The complete genome of DAR4145 provides a valuable resource for future investigations into the global dissemination and phylogeography of ST772-MRSA-V.

Keywords Staphylococcus aureus
MRSA
ST772
Antibiotic resistance
Mobile genetic elements
Complete genome
DAR4145
India
DOI http://dx.doi.org/10.1186/s12864-015-1599-9   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 4.0 License
URL https://creativecommons.org/licenses/by/4.0/au


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