Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia

Ginther, Jennifer L., Mayo, Mark J., Warrington, Stephanie D., Kaestli, Mirjam E., Mullins, Travis, Wagner, David M., Currie, Bart J., Tuanyok, Apichai and Keim, Paul (2015). Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. PLoS Neglected Tropical Diseases,9(6).

Document type: Journal Article
Citation counts: Altmetric Score Altmetric Score is 1
Google Scholar Search Google Scholar
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Mayo_59088.pdf Published version application/pdf 2.68MB 7
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

IRMA ID 11381xPUB120
Title Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia
Author Ginther, Jennifer L.
Mayo, Mark J.
Warrington, Stephanie D.
Kaestli, Mirjam E.
Mullins, Travis
Wagner, David M.
Currie, Bart J.
Tuanyok, Apichai
Keim, Paul
Journal Name PLoS Neglected Tropical Diseases
Publication Date 2015
Volume Number 9
Issue Number 6
ISSN 1935-2735   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84934779506
Total Pages 13
Place of Publication United States of America
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract Identification and characterization of near-neighbor species are critical to the development of robust molecular diagnostic tools for biothreat agents. One such agent, Burkholderia pseudomallei, a soil bacterium and the causative agent of melioidosis, is lacking in this area because of its genomic diversity and widespread geographic distribution. The Burkholderia genus contains over 60 species and occupies a large range of environments including soil, plants, rhizospheres, water, animals and humans. The identification of novel species in new locations necessitates the need to identify the true global distribution of Burkholderia species, especially the members that are closely related to B. pseudomallei. In our current study, we used the Burkholderia-specific recA sequencing assay to analyze environmental samples from the Darwin region in the Northern Territory of Australia where melioidosis is endemic. Burkholderia recA PCR negative samples were further characterized using 16s rRNA sequencing for species identification. Phylogenetic analysis demonstrated that over 70% of the bacterial isolates were identified as B. ubonensis indicating that this species is common in the soil where B. pseudomallei is endemic. Bayesian phylogenetic analysis reveals many novel branches within the B. cepacia complex, one novel B. oklahomensis-like species, and one novel branch containing one isolate that is distinct from all other samples on the phylogenetic tree. During the analysis with recA sequencing, we discovered 2 single nucleotide polymorphisms in the reverse priming region of B. oklahomensis. A degenerate primer was developed and is proposed for future use. We conclude that the recA sequencing technique is an effective tool to classify Burkholderia and identify soil organisms in a melioidosis endemic area.
DOI http://dx.doi.org/10.1371/journal.pntd.0003892   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 4.0 License
URL https://creativecommons.org/licenses/by/4.0/au


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 8 Abstract Views, 7 File Downloads  -  Detailed Statistics
Created: Tue, 26 Jul 2016, 12:45:26 CST