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Improving immunity to Haemophilus influenzae in children with chronic suppurative lung disease

Pizzutto, Susan J., Yerkovich, Stephanie T., Upham, John W., Hales, Belinda J., Thomas, Wayne R. and Chang, Anne B. (2015). Improving immunity to Haemophilus influenzae in children with chronic suppurative lung disease. Vaccine,33(2):321-326.

Document type: Journal Article
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IRMA ID 84473293xPUB134
NHMRC Grant No. 1038415
1040830
Title Improving immunity to Haemophilus influenzae in children with chronic suppurative lung disease
Author Pizzutto, Susan J.
Yerkovich, Stephanie T.
Upham, John W.
Hales, Belinda J.
Thomas, Wayne R.
Chang, Anne B.
Journal Name Vaccine
Publication Date 2015
Volume Number 33
Issue Number 2
ISSN 0264-410X   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84922357332
Start Page 321
End Page 326
Total Pages 6
Place of Publication United Kingdom
Publisher Elsevier Ltd
HERDC Category C1 - Journal Article (DIISR)
Abstract Background

Endobronchial infections related to non-typeable Haemophilus influenzae (NTHi) are common in children and adults with suppurative airway disease such as bronchiectasis and COPD. Impaired cell mediated immune responses to NTHi have been described in these patients. Currently there are no interventions known to correct the deficiency in cell mediated immune responses to NTHi. The aim of this study was to determine if receipt of a conjugate vaccine containing protein D from H. influenzae is associated with improvement in NTHi-specific cytokine responses in children with chronic suppurative lung disease.

Methods

Blood mononuclear cells from 107 young children with chronic suppurative lung disease and 32 healthy control children were stimulated in vitro with NTHi. We compared the cytokine production of stimulated mononuclear cells from children who had received the pneumococcal H. influenzae protein D conjugate vaccine with cells from children who received pneumococcal vaccines without protein D. Protein D-specific IgG1 was quantified in plasma.

Results

Children with chronic suppurative lung disease who received ≥3 doses of the protein D conjugate vaccine produced significantly more IFNγ than children who received the alternative vaccines without protein D (median 939 versus 338 pg/ml; p = 0.007). Importantly, the amount of IFNγ produced by those vaccinated with the conjugate vaccine approached the levels observed in cells from healthy children. The conjugate vaccine was also associated with small but significant increases in IL-13 (p < 0.001) and IL-5 (p = 0.007). Protein D-specific IgG1 levels correlated with the number of PHiD-CV doses (p = 0.02).

Conclusion

Vaccination with PHiD-CV is associated with improvements in NTHi-specific cell-mediated and humoral immune responses in children with chronic suppurative lung disease.
Keywords Pneumococcal H. influenzae protein-D conjugate vaccine
Cell mediated immune response
Non-CF bronchiectasis
Non-typeable Haemophilus influenzae
DOI http://dx.doi.org/10.1016/j.vaccine.2014.11.024   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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