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Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting

Tong, Steven Y. C., Holden, Matthew T.G., Nickerson, Emma K., Cooper, Ben S., Köser, Claudio U., Cori, Anne, Jombart, Thibaut, Cauchemez, Simon, Fraser, Christophe, Wuthiekanun, Vanaporn, Thaipadungpanit, Janjira, Hongsuwan, Maliwan, Day, Nicholas P. J., Limmathurotsakul, Direk, Parkhill, Julian and Peacock, Sharon J. (2015). Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting. Genome Research,25:111-118.

Document type: Journal Article
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IRMA ID 84473293xPUB141
NHMRC Grant No. 1065736
Title Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting
Author Tong, Steven Y. C.
Holden, Matthew T.G.
Nickerson, Emma K.
Cooper, Ben S.
Köser, Claudio U.
Cori, Anne
Jombart, Thibaut
Cauchemez, Simon
Fraser, Christophe
Wuthiekanun, Vanaporn
Thaipadungpanit, Janjira
Hongsuwan, Maliwan
Day, Nicholas P. J.
Limmathurotsakul, Direk
Parkhill, Julian
Peacock, Sharon J.
Journal Name Genome Research
Publication Date 2015
Volume Number 25
ISSN 1088-9051   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84920661654
Start Page 111
End Page 118
Total Pages 8
Place of Publication United States of America
Publisher Cold Spring Harbor Laboratory Press
HERDC Category C1 - Journal Article (DIISR)
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infection. Whole-genome sequencing of MRSA has been used to define phylogeny and transmission in well-resourced healthcare settings, yet the greatest burden of nosocomial infection occurs in resource-restricted settings where barriers to transmission are lower. Here, we study the flux and genetic diversity of MRSA on ward and individual patient levels in a hospital where transmission was common. We repeatedly screened all patients on two intensive care units for MRSA carriage over a 3-mo period. All MRSA belonged to multilocus sequence type 239 (ST 239). We defined the population structure and charted the spread of MRSA by sequencing 79 isolates from 46 patients and five members of staff, including the first MRSA-positive screen isolates and up to two repeat isolates where available. Phylogenetic analysis identified a flux of distinct ST 239 clades over time in each intensive care unit. In total, five main clades were identified, which varied in the carriage of plasmids encoding antiseptic and antimicrobial resistance determinants. Sequence data confirmed intra- and interwards transmission events and identified individual patients who were colonized by more than one clade. One patient on each unit was the source of numerous transmission events, and deep sampling of one of these cases demonstrated colonization with a “cloud” of related MRSA variants. The application of whole-genome sequencing and analysis provides novel insights into the transmission of MRSA in under-resourced healthcare settings and has relevance to wider global health.
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Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 4.0 License

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