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Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1

Mittag, Diana, Sran, Amrita, Chan, Kasey S., Boland, Martin P., Bandala-Sanchez, Ester, Huet, Olivier, Xu, William and Sparrow, Rosemary L. (2015). Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1. Transfusion,55(9):2197-2206.

Document type: Journal Article
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IRMA ID 84377429xPUB68
Title Stored red blood cell susceptibility to in vitro transfusion-associated stress conditions is higher after longer storage and increased by storage in saline-adenine-glucose-mannitol compared to AS-1
Author Mittag, Diana
Sran, Amrita
Chan, Kasey S.
Boland, Martin P.
Bandala-Sanchez, Ester
Huet, Olivier
Xu, William
Sparrow, Rosemary L.
Journal Name Transfusion
Publication Date 2015
Volume Number 55
Issue Number 9
ISSN 0041-1132   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84941802970
Start Page 2197
End Page 2206
Total Pages 10
Place of Publication United States
Publisher Wiley-Blackwell Publishing, Inc.
Field of Research MEDICAL AND HEALTH SCIENCES
HERDC Category C1 - Journal Article (DIISR)
Abstract Background
Biochemical changes induced in red blood cells (RBCs) during storage may impair their function upon transfusion. Transfusion-associated stresses may further amplify storage lesion effects including increased phosphatidylserine (PS)-exposure at the RBC membrane, microparticle (MP)-release and adhesion to endothelial cells (ECs). RBC stress susceptibility in vitro was investigated in relation to storage time and additive solution.

Study design and methods

Leukocyte-reduced whole blood donations (n=18) were paired, mixed and re-split before separating the RBCs for storage in SAGM or AS-1 solutions. Samples were taken after 3, 21 or 35 days. For oxidative stress treatment RBCs were exposed to 0.5mM tert-butylhydroperoxide. Transfusion-associated stress was simulated by overnight culture at 37 °C with plasma containing inflammatory mediators. PS-exposure and MPs were measured by flow cytometry and adhesion to EC was tested under flow conditions. PS specificity of adhesion was tested by blocking with PS-containing lipid vesicles.

Results

Oxidative stress induced significantly higher PS-exposure and adhesion to ECs in RBCs stored for 35 days compared to 3 days (p<0.04). PS-containing vesicles blocked RBC-EC adhesion. After overnight culture with or without plasma, PS-exposure and EC-adhesion were significantly increased (p<0.05). MP numbers increased with longer RBC storage and after RBC culture with plasma. Culture conditions influenced MP numbers from day 35 RBCs. RBCs stored in SAGM had significantly higher PS-exposure after stress treatment than AS-1 RBCs (p<0.02).

Conclusion

Storage for 35 days significantly increased RBC susceptibility to oxidative and in vitro transfusion-associated stresses and was higher for RBCs stored in SAGM compared to AS-1
Keywords Red blood cells
storage lesion
oxidative stress
stress susceptibility
transfusion
phosphatidylserine
adhesion
AS-1
SAGM
microparticles
DOI http://dx.doi.org/10.1111/trf.13138   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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