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Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus)

Milic, Natalie L., Davis, Stephen, Carr, Jillian M., Isberg, Sally, Beard, Michael R. and Helbig, Karla J. (2015). Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus). Developmental and Comparative Immunology,51(1):108-115.

Document type: Journal Article
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IRMA ID 75039815xPUB808
Title Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus)
Author Milic, Natalie L.
Davis, Stephen
Carr, Jillian M.
Isberg, Sally
Beard, Michael R.
Helbig, Karla J.
Journal Name Developmental and Comparative Immunology
Publication Date 2015
Volume Number 51
Issue Number 1
ISSN 0145-305X   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84924952113
Start Page 108
End Page 115
Total Pages 8
Place of Publication United Kingdom
Publisher Pergamon Press
HERDC Category C1 - Journal Article (DIISR)
Abstract A number of pathogens have been detected in crocodiles, however little is known about their ability to control these pathogens. The interferon stimulated gene (ISG), viperin, has gained attention recently as an important host protein involved in multiple arms of the immune response. Viperin in concert with a number of other ISGs was upregulated in response to viral nucleic acid mimics and sendai virus in the C. porosus cell line, LV-1, indicating an intact early innate response to viral infection in these animals for the first time. Viperin was cloned from the LV-1 cell line and shown to have similar localisation patterns as human viperin, as well as demonstrating extremely high conservation with the human orthologue, excepting at the N-terminus. Interestingly, C. porosus viperin was also able to inhibit Dengue virus replication in vitro, showing a high level of intact functionality for this protein across divergent animal species, and perhaps demonstrating its importance in the early innate response to pathogens in the animal kingdom.
Keywords Viperin
Innate immunity
Interferon stimulated gene
Antiviral function
Crocodile
DOI http://dx.doi.org/10.1016/j.dci.2015.03.001   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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