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Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom

Herzig, Volker, Khalife, Ali A., Chong, Youmie, Isbister, Geoffrey K., Currie, Bart J., Churchill, Tracey B., Horner, Suzanne, Escoubas, Pierre, Nicholson, Graham M. and Hodgson, Wayne C. (2008). Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom. Toxicon,51(7):1167-1177.

Document type: Journal Article

IRMA ID 10139xPUB129
Title Intersexual variations in Northern (Missulena pruinosa) and Eastern (M. bradleyi) mouse spider venom
Author Herzig, Volker
Khalife, Ali A.
Chong, Youmie
Isbister, Geoffrey K.
Currie, Bart J.
Churchill, Tracey B.
Horner, Suzanne
Escoubas, Pierre
Nicholson, Graham M.
Hodgson, Wayne C.
Journal Name Toxicon
Publication Date 2008
Volume Number 51
Issue Number 7
ISSN 0041-0101   (check CDU catalogue  open catalogue search in new window)
Start Page 1167
End Page 1177
Total Pages 11
Place of Publication UK
Publisher Pergamon
Field of Research 1115 - Pharmacology and Pharmaceutical Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract Venoms of both sexes of Australian Northern (Missulena pruinosa) and Eastern (Missulena bradleyi) mouse spiders were studied in order to determine intersexual variations in venom yield, composition and bioactivity. Females of both species yielded more venom than males. High-performance liquid chromatography (HPLC) and mass spectrometry data further indicate a substantial degree of intersexual variation in the venom composition of both species. In a cricket (Acheta domestica) acute toxicity assay, only small intersexual differences were observed, but M. bradleyi venom was found to be considerably more potent than M. pruinosa venom. In the chick biventer cervicis nerve–muscle preparation, male but not female M. bradleyi venom induced large and sustained muscle contractions with fasciculation and decreased twitch height that could be reversed by CSL funnel-web spider antivenom. In contrast, venoms of both sexes of M. pruinosa did not induce significant effects in the chick biventer cervicis nerve–muscle preparation. We therefore conclude that female M. bradleyi venom and venoms from male and female M. pruinosa appear to contain few, if any, orthologs of δ-missulenatoxin-Mb1a, the toxin responsible for the effects of male M. bradleyi venom in vertebrates. These findings are consistent with clinical reports that mouse spiders, particularly species other than male M. bradleyi, do not appear to be a major medical problem in humans.
Keywords Missulena pruinosa
Missulena bradleyi
Mouse spider
Actinopodidae
Venom
Intersexual variations
DOI http://dx.doi.org/10.1016/j.toxicon.2008.02.001   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Wed, 15 Apr 2009, 16:18:29 CST by Sarena Wegener