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Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia

Kumar, A., Mayo, Mark J., Trunck, L., Cheng, Allen C., Currie, Bart J. and Schweizer, H. (2008). Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia. Transactions of the Royal Society of Tropical Medicine and Hygiene,102(Suppl. 1):S145-S151.

Document type: Journal Article

IRMA ID 10103xPUB18
Title Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia
Author Kumar, A.
Mayo, Mark J.
Trunck, L.
Cheng, Allen C.
Currie, Bart J.
Schweizer, H.
Journal Name Transactions of the Royal Society of Tropical Medicine and Hygiene
Publication Date 2008
Volume Number 102
Issue Number Suppl. 1
ISSN 0035-9203   (check CDU catalogue open catalogue search in new window)
Start Page S145
End Page S151
Total Pages 1
Place of Publication UK
Publisher Elsevier
Field of Research 0605 - Microbiology
1108 - Medical Microbiology
1117 - Public Health and Health Services
HERDC Category C1 - Journal Article (DEST)
Abstract Burkholderia pseudomallei is the aetiological agent of melioidosis. Therapy for this disease is lengthy and limited to only a few antibiotics because of this bacterium's intrinsic antibiotic resistance to many clinically useful antibiotics. These properties of B. pseudomallei may partially be due to expression of efflux pumps of the resistance-nodulation-cell-division (RND) family. The patterns and magnitude of RND efflux pump expression in commonly used strains and clinical isolates of B. pseudomallei from the Royal Darwin Hospital, Darwin, Australia, were assessed in cells grown to late exponential phase using quantitative real-time PCR (qRT-PCR). Expression of the three previously identified RND efflux pumps AmrAB-OprA, BpeAB-OprB and BpeEF-OprC, as well as four other yet uncharacterized pumps, was found to be widespread in the clinical isolates. In 45 of 50 isolates (90%), mRNA was detected for at least one of the seven RND pumps. Of these 45 isolates, 41 (82%) expressed multiple pumps with nine strains expressing all seven pumps tested. While these studies revealed no striking correlation between RND efflux pump expression and clinically significant antibiotic resistance, the data support the notion that RND pumps probably play important roles in this bacterium's physiology, defence against toxic compounds, and perhaps virulence.
DOI http://dx.doi.org/10.1016/S0035-9203(08)70032-4   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Tue, 28 Apr 2009, 15:02:30 CST by Sarena Wegener