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High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites

Pasay, C, Arlian, L, Morgan, M, Vyszenski-moher, D, Rose, Alea, Holt, Deborah, Walton, S and Mccarthy, J (2008). High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites. Medical and Veterinary Entomology,22(1):82-88.

Document type: Journal Article

IRMA ID 10239xPUB17
Title High-resolution melt analysis for the detection of a mutation associated with permethrin resistance in a population of scabies mites
Author Pasay, C
Arlian, L
Morgan, M
Vyszenski-moher, D
Rose, Alea
Holt, Deborah
Walton, S
Mccarthy, J
Journal Name Medical and Veterinary Entomology
Publication Date 2008
Volume Number 22
Issue Number 1
ISSN 0269-283X   (check CDU catalogue open catalogue search in new window)
Start Page 82
End Page 88
Total Pages 7
Place of Publication UK
Publisher Wiley-Blackwell Publishing Asia
Field of Research 0699 - Other Biological Sciences
0799 - Other Agricultural and Veterinary Sciences
1103 - Clinical Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract Permethrin as a topical acaricide cream is widely used to treat scabies. The neuronal voltage-sensitive sodium channel (Vssc), necessary for the generation of action potentials in excitable cells, is the target of pyrethroid acaricides such as permethrin. Pyrethroid resistance has been linked to specific mutations in the Vssc gene. Following the partial sequencing of the Vssc gene in the scabies mite Sarcoptes scabiei (L.) (Astigmata: Sarcoptidae), we compared Vssc gene sequences from permethrin-sensitive and -tolerant S. scabiei var. canis Gerlach mites, and identified a G to A single nucleotide polymorphism (SNP) in permethrin-tolerant mites resulting in an amino acid change from glycine to aspartic acid in domain III S6. The mutation is in a region of the gene where mutations have been identified in a range of pyrethroid-resistant arthropods. Results of in vitro permethrin exposure assays showed that survival rates for mites bearing the mutation were similar to those previously reported for mites from human subjects where clinical tolerance to permethrin had been observed. A real-time polymerase chain reaction−high-resolution melt (PCR-HRM) assay was developed to detect this SNP. This assay provides a useful methodology for screening for this and other mutations associated with permethrin resistance in scabies mite populations and thus facilitates surveillance for acaricide resistance.
DOI http://dx.doi.org/10.1111/j.1365-2915.2008.00716.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Wed, 29 Apr 2009, 13:06:21 CST by Sarena Wegener