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Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling

Baranski, M., Rourke, M., Loughnan, S., Hayes, B., Austin, Christopher M. and Robinson, Nick (2008). Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling. Animal Genetics,39(6):606-614.

Document type: Journal Article

IRMA ID 78674128xPUB34
Title Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling
Author Baranski, M.
Rourke, M.
Loughnan, S.
Hayes, B.
Austin, Christopher M.
Robinson, Nick
Journal Name Animal Genetics
Publication Date 2008
Volume Number 39
Issue Number 6
ISSN 0268-9146   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-55949113046
Start Page 606
End Page 614
Total Pages 9
Place of Publication France
Publisher International Society for Animal Genetics
Field of Research 0707 - Veterinary Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract The objective of this study was to identify QTL for growth rate in the blacklip abalone Haliotis rubra using selective DNA pooling. Three full-sibling families of H. rubra derived from crosses of wild broodstock were used. DNA was extracted from the largest and smallest 10% of progeny and combined into two pools for each phenotypic tail. The DNA pools were typed with 139 microsatellites, and markers showing significant differences between the peak height ratios of alleles inherited from the parents were individually genotyped and analysed by interval mapping. A strong correlation (r = 0.94, P < 0.001) was found between the t-values from the analysis of pools and the t-values from the analysis of individual genotypes. Based on the interval mapping analysis, QTL were detected on nine linkage groups at a chromosome-wide P < 0.01 and one linkage group at a chromosome-wide P < 0.05. The study demonstrated that selective DNA pooling is efficient and effective as a first-pass screen for the discovery of QTL in an aquaculture species.
DOI http://dx.doi.org/10.1111/j.1365-2052.2008.01773.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Fri, 08 May 2009, 10:04:24 CST by Sarena Wegener