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Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis

Andersson, Patiyan, Harris, Tegan M., Tong, Steven Y. C. and Giffard, Philip M. (2009). Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis. Antimicrobial Agents and Chemotherapy,53(6):2679-2683.

Document type: Journal Article
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IRMA ID 75039815xPUB33
Title Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis
Author Andersson, Patiyan
Harris, Tegan M.
Tong, Steven Y. C.
Giffard, Philip M.
Journal Name Antimicrobial Agents and Chemotherapy
Publication Date 2009
Volume Number 53
Issue Number 6
ISSN 1098-6596   (check CDU catalogue open catalogue search in new window)
Start Page 2679
End Page 2683
Total Pages 5
Place of Publication Washington, United States
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.
DOI http://dx.doi.org/10.1128/AAC.00063-09   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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