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Landscape Changes Influence the Occurrence of the Melioidosis Bacterium Burkholderia pseudomallei in Soil in Northern Australia

Kaestli, Mirjam, Mayo, Mark, Harrington, Glenda, Ward, Linda, Watt, Felicity, Hill, Jason V., Cheng, Allen C. and Currie, Bart J. (2009). Landscape Changes Influence the Occurrence of the Melioidosis Bacterium Burkholderia pseudomallei in Soil in Northern Australia. PLoS Neglected Tropical Diseases,3(1):e364.

Document type: Journal Article
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Title Landscape Changes Influence the Occurrence of the Melioidosis Bacterium Burkholderia pseudomallei in Soil in Northern Australia
Author Kaestli, Mirjam
Mayo, Mark
Harrington, Glenda
Ward, Linda
Watt, Felicity
Hill, Jason V.
Cheng, Allen C.
Currie, Bart J.
Journal Name PLoS Neglected Tropical Diseases
Publication Date 2009
Volume Number 3
Issue Number 1
ISSN 1935-2735   (check CDU catalogue  open catalogue search in new window)
Start Page e364
Total Pages 8
Place of Publication San Francisco, CA, United States
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DEST)
Abstract Background
The soil-dwelling saprophyte bacterium Burkholderia pseudomallei is the cause of melioidosis, a severe disease of humans and animals in southeast Asia and northern Australia. Despite the detection of B. pseudomallei in various soil and water samples from endemic areas, the environmental habitat of B. pseudomallei remains unclear.

Methodology/Principal Findings

We performed a large survey in the Darwin area in tropical Australia and screened 809 soil samples for the presence of these bacteria. B. pseudomallei were detected by using a recently developed and validated protocol involving soil DNA extraction and real-time PCR targeting the B. pseudomallei–specific Type III Secretion System TTS1 gene cluster. Statistical analyses such as multivariable cluster logistic regression and principal component analysis were performed to assess the association of B. pseudomallei with environmental factors. The combination of factors describing the habitat of B. pseudomallei differed between undisturbed sites and environmentally manipulated areas. At undisturbed sites, the occurrence of B. pseudomallei was found to be significantly associated with areas rich in grasses, whereas at environmentally disturbed sites, B. pseudomallei was associated with the presence of livestock animals, lower soil pH and different combinations of soil texture and colour.

Conclusions/Significance

This study contributes to the elucidation of environmental factors influencing the occurrence of B. pseudomallei and raises concerns that B. pseudomallei may spread due to changes in land use.
Keywords landscape
Melioidosis Bacterium
Burkholderia pseudomallei
soil
northern Australia
DOI http://dx.doi.org/10.1371/journal.pntd.0000364   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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