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Detection of Phytoplasmas in Declining Pears in Southern Australia

Schneider, BL and Gibb, KS (1997). Detection of Phytoplasmas in Declining Pears in Southern Australia. Plant Disease,81(3):254-258.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 22 times in Scopus Article | Citations

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Title Detection of Phytoplasmas in Declining Pears in Southern Australia
Author Schneider, BL
Gibb, KS
Journal Name Plant Disease
Publication Date 1997
Volume Number 81
Issue Number 3
ISSN 0191-2917   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0030971919
Start Page 254
End Page 258
Total Pages 5
Place of Publication USA
Publisher American Phytopathological Society
HERDC Category C1 - Journal Article (DEST)
Abstract Forty-nine pear tree samples collected in Victoria, most of them showing decline symptoms, were tested by polymerase chain reaction (PCR) analysis to detect phytoplasmas. Two universal phytoplasma-specific primer pairs, fP1/rP7 and fU5/rU3, were tested, but only fU5/rU3 amplified the phytoplasma DNA adequately. Nested PCR with universal and group-specific primers, however, proved more effective. Thirty pear trees reacted positively in a nested PCR assay. Restriction fragment length polymorphism (RFLP) analysis with the restriction enzymes MseI and AluI of the PCR fragment amplified with the primer pair fU5/rU3 revealed patterns identical to those from the sweet potato little leaf phytoplasma. This is the first report of a phytoplasma in pear in Australia.
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Created: Fri, 12 Sep 2008, 08:35:25 CST by Administrator