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Detection and differentiation of phytoplasmas in Australia

Davis, RI, Schneider, BL and Gibb, KS (1997). Detection and differentiation of phytoplasmas in Australia. Australian Journal of Agricultural Research,48(5):535-544.

Document type: Journal Article
Citation counts: TR Web of Science Citation Count  Cited 47 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 52 times in Scopus Article | Citations

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ISI LOC A1997XC74900001
Title Detection and differentiation of phytoplasmas in Australia
Author Davis, RI
Schneider, BL
Gibb, KS
Journal Name Australian Journal of Agricultural Research
Publication Date 1997
Volume Number 48
Issue Number 5
ISSN 0004-9409   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0030905832
Start Page 535
End Page 544
Total Pages 10
Place of Publication Australia
Publisher CSIRO Publishing
HERDC Category C1 - Journal Article (DEST)
Abstract In a polymerase chain reaction (PCR) diagnostic test, phytoplasma (formerly known as plant-pathogenic mycoplasma-like organism or MLO) ribosomal DNA was detected in total DNA extracts prepared from 56 out of 63 plants collected from geographically diverse locations across Australia. The list of phytoplasma hosts consisted of 38 di®erent species in 16 di®erent families. Restriction site analysis of the PCR-ampli¯ed DNA accessions was used to divide the phytoplasmas into 2 groups. The majority of the tomato big bud group and sweet potato little leaf group phytoplasmas were closely related to a phytoplasma originally obtained from Crotalaria in Thailand, which is a member of the faba bean phyllody strain cluster. In contrast, phytoplasmas associated with Australian grapevine yellows and papaya dieback were most similar to members of the aster yellows strain cluster. Twelve phytoplasmas were compared by Southern blot hybridisation with DNA cloned from the sweet potato little leaf phytoplasma strain V4. The restriction fragment length polymorphism pattern of all phytoplasmas compared was identical except for 2 sweet potato little leaf phytoplasmas.
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