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Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas

Schneider, BL, Gibb, KS and Seemuller, E (1997). Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology,143(10):3381-3389.

Document type: Journal Article
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Title Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas
Author Schneider, BL
Gibb, KS
Seemuller, E
Journal Name Microbiology
Publication Date 1997
Volume Number 143
Issue Number 10
ISSN 1350-0872   (check CDU catalogue open catalogue search in new window)
Start Page 3381
End Page 3389
Total Pages 9
Place of Publication Great Britain
Publisher Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Primers designed from sequences of the gene encoding the elongation factor Tu (tuf gene) of several culturable mollicutes amplified most of the tuf gene from phytoplasmas of the aster yellows, stolbur and X-disease groups. About 85% of the tuf gene from two aster yellows strains and a tomato stolbur phytoplasma was sequenced. The nucleotide sequence similarity between these related phytoplasmas was between 87.8 and 97.0%, whereas the homology with other mollicutes was 66.3-72.7%. The similarity of the deduced amino acid sequence was significantly higher, ranging from 96.0 to 99.4% among the phytoplasmas and 78.5% to 83.3% between phytoplasmas and the culturable mollicutes examined. From the nucleotide sequences of the phytoplasma strains, two pairs of primers were designed; one amplified the phytoplasmas of most phylogenetic groups that were established, the other was specific for the aster yellows and stolbur groups. The phytoplasmas of the various groups that were amplified could be distinguished by RFLP analysis using Sau3AI, AluI and HpaII. The aster yellows group could be divided into five Sau3AI RFLP groups. These results showed that the tuf gene has the potential to be used to differentiate and classify phytoplasmas. Southern blot analysis revealed that the tuf gene is present as a single copy.
 
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