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So you need a protein - A guide to the production of recombinant proteins

Jayaraj, Ramamoorthi and Smooker, Peter M. (2009). So you need a protein - A guide to the production of recombinant proteins. Open Veterinary Science Journal,3:28-34.

Document type: Journal Article
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Title So you need a protein - A guide to the production of recombinant proteins
Author Jayaraj, Ramamoorthi
Smooker, Peter M.
Journal Name Open Veterinary Science Journal
Publication Date 2009
Volume Number 3
ISSN 1874-3188   (check CDU catalogue open catalogue search in new window)
Start Page 28
End Page 34
Total Pages 7
Place of Publication Bussum, Netherlands
Publisher Bentham Open
HERDC Category C1 - Journal Article (DEST)
Abstract The field of biotechnology owes a great deal to the ability to produce recombinant proteins, which can be made in far greater abundance than many native proteins, and are more easily quality controlled. There is a great need for individual proteins to be produced for research purposes. This review is aimed at researchers who are not experienced at protein expression, but find that they have a need to produce a recombinant protein. We detail the major expression systems that will be commonly used in the laboratory situation- bacterial, yeast and insect cell culture. The application of each, and the relative advantages/disadvantages are discussed.
Keywords biotechnology
recombinant proteins
native proteins
protein expression
bacteria culture
yeast culture
insect cell culture
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