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Application of a Method for Obtaining Lymph from Anuran Amphibians

Reynolds, Stephen J., Christian, Keith A. and Tracy, Christopher R. (2009). Application of a Method for Obtaining Lymph from Anuran Amphibians. Journal of Herpetology,43(1):148-153.

Document type: Journal Article

IRMA ID 80801157xPUB25
Title Application of a Method for Obtaining Lymph from Anuran Amphibians
Author Reynolds, Stephen J.
Christian, Keith A.
Tracy, Christopher R.
Journal Name Journal of Herpetology
Publication Date 2009
Volume Number 43
Issue Number 1
ISSN 0022-1511   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-77649263772
Start Page 148
End Page 153
Total Pages 6
Place of Publication US
Publisher Allen Press
HERDC Category C1 - Journal Article (DEST)
Abstract Methods for obtaining plasma from anurans are unsatisfactory in that they are inadequately described, are difficult to perform, or are lethal to the subject. A lymph extraction technique involving temporary ligation is described that enables sufficient fluid to be obtained for analysis from frogs greater than 20 g. Lymph was more easily obtained from Bufo marinus than from the austral-hylids Litoria caerulea and Cyclorana australis. Lymph accumulation was faster in hydrated frogs or those with full bladders, whereas it was relatively slow in animals that had been dormant or that were dehydrated. Differences between the osmolality of lymph and blood-plasma were variable but slight; presumably the relatively high endothelial permeability of amphibians facilitates significant fluid exchange.
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Created: Wed, 24 Mar 2010, 15:14:23 CST by Sarena Wegener