Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Genomic location and variation of the gene for CRS, a complement binding protein in the M57 strains of Streptococcus pyogenes

Binks, Michael J., McMillan, David J. and Sriprakash, Kadaba S. (2003). Genomic location and variation of the gene for CRS, a complement binding protein in the M57 strains of Streptococcus pyogenes. Infection and Immunity,71(12):6701-6706.

Document type: Journal Article
Citation counts:
Google Scholar Search Google Scholar
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Binks_8896.pdf Published version application/pdf 606.69KB 33
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

Title Genomic location and variation of the gene for CRS, a complement binding protein in the M57 strains of Streptococcus pyogenes
Author Binks, Michael J.
McMillan, David J.
Sriprakash, Kadaba S.
Journal Name Infection and Immunity
Publication Date 2003
Volume Number 71
Issue Number 12
ISSN 1098-5522   (check CDU catalogue  open catalogue search in new window)
Start Page 6701
End Page 6706
Total Pages 6
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 0605 - Microbiology
1103 - Clinical Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract All isolates of serotype M1 of group A streptococci possess a gene for streptococcal inhibitor of complement (SIC) in the mga regulon, which harbors genes for other virulence factors, such as M and M-like proteins, C5a peptidase, and a regulator. In serotype M57 the gene for a protein that is closely related to SIC (crs57) is located outside the mga regulon. We mapped the location of the crs57 gene in six strains of emm57 (gene encoding the M57 protein) sequence types to an intergenic region between the ABC transporter gene (SPy0778) and the gene for a small ribosomal protein (rpsU). The noncoding sequences on both sides of crs57 exhibited high degrees of identity to the corresponding regions of sic from M1 strains. This included one of the inverted repeat sequences of IS1562 but not the insertion element itself. These observations suggest that crs57 was recently acquired by serotype M57 or its progenitor via horizontal acquisition from serotype M1. The six emm57 sequence type isolates analyzed in this study belong to two distinct molecular types (vir types VT8 and VT101). Although the crs57 sequences from VT8 strains had very few substitution mutations, the VT101 crs57 sequence had a large number of such mutations. The CRS57 proteins from these strains are secretory products and have the ability to bind to complement proteins. All these proteins contain several tryptophan-rich repeats designated DWS motifs and internal repeat sequences. In all of these structural and biochemical characteristics CRS57 resembles SIC from M1 strains. Hence, CRS57 has a functional role similar to that of SIC in an M1 strain.
DOI http://dx.doi.org/10.1128/IAI.71.12.6701-6706.2003   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright by the American Society for Microbiology


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 39 Abstract Views, 34 File Downloads  -  Detailed Statistics
Created: Sun, 11 Apr 2010, 00:56:47 CST by Sarena Wegener