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High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni

Price, Erin P., Smith, Helen, Huygens, Flavia and Giffard, Philip M. (2007). High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni. Applied and Environmental Microbiology,73(10):3431-3436.

Document type: Journal Article
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IRMA ID 10603xPUB38
Title High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni
Author Price, Erin P.
Smith, Helen
Huygens, Flavia
Giffard, Philip M.
Journal Name Applied and Environmental Microbiology
Publication Date 2007
Volume Number 73
Issue Number 10
ISSN 0099-2240   (check CDU catalogue open catalogue search in new window)
Start Page 3431
End Page 3436
Total Pages 6
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 0604 - Genetics
0799 - Other Agricultural and Veterinary Sciences
1004 - Medical Biotechnology
HERDC Category C1 - Journal Article (DEST)
Abstract A novel method for genotyping the clustered regularly interspaced short palindromic repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods, and emphasizes the potential of HRM for genotyping short sequence repeats in other species.
Keywords Campylobacter jejuni
DOI http://dx.doi.org/10.1128/AEM.02702-06   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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