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Inactivation of an iron transporter in Lactococcus lactis results in resistance to tellurite and oxidative Stress

Turner, Mark S., Tan, Yu Pei and Giffard, Philip M. (2007). Inactivation of an iron transporter in Lactococcus lactis results in resistance to tellurite and oxidative Stress. Applied and Environmental Microbiology,73(19):6144-6149.

Document type: Journal Article
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IRMA ID 10603xPUB34
Title Inactivation of an iron transporter in Lactococcus lactis results in resistance to tellurite and oxidative Stress
Author Turner, Mark S.
Tan, Yu Pei
Giffard, Philip M.
Journal Name Applied and Environmental Microbiology
Publication Date 2007
Volume Number 73
Issue Number 19
ISSN 0099-2240   (check CDU catalogue open catalogue search in new window)
Start Page 6144
End Page 6149
Total Pages 6
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 0605 - Microbiology
0799 - Other Agricultural and Veterinary Sciences
1004 - Medical Biotechnology
HERDC Category C1 - Journal Article (DEST)
Abstract In Lactococcus lactis, the interactions between oxidative defense, metal metabolism, and respiratory metabolism are not fully understood. To provide an insight into these processes, we isolated and characterized mutants of L. lactis resistant to the oxidizing agent tellurite (TeO32–), which generates superoxide radicals intracellularly. A collection of tellurite-resistant mutants was obtained using random transposon mutagenesis of L. lactis. These contained insertions in genes encoding a proton-coupled Mn2+/Fe2+ transport homolog (mntH), the high-affinity phosphate transport system (pstABCDEF), a putative osmoprotectant uptake system (choQ), and a homolog of the oxidative defense regulator spx (trmA). The tellurite-resistant mutants all had better survival than the wild type following aerated growth. The mntH mutant was found to be impaired in Fe2+ uptake, suggesting that MntH is a Fe2+ transporter in L. lactis. This mutant is capable of carrying out respiration but does not generate as high a final pH and does not exhibit the long lag phase in the presence of hemin and oxygen that is characteristic of wild-type L. lactis. This study suggests that tellurite-resistant mutants also have increased resistance to oxidative stress and that intracellular Fe2+ can heighten tellurite and oxygen toxicity.
Keywords Lactococcus lactis
DOI http://dx.doi.org/10.1128/AEM.00413-07   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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